Method for automated structuring of product data and its applications

Product structures represent the data backbone for through-life management of complex systems. Product Lifecycle Management (PLM) Systems are used to maintain product structures and track product changes. However, in maintenance, repair and overhaul (MRO) product composition often is unknown when MRO service providers are not the original manufacturers. Thus, MRO processes start with an exhaustive product diagnosis to identify elements to be maintained or replaced. Existing 3D scanning and data post processing methods have to be improved to acquire structured product data. This paper presents a method for automated derivation of product structures from 3D assembly models

Design of graphite and the Polyhedral Compilation Package

Graphite is the loop transformation framework that was introduced in GCC 4.4. This paper gives a detailed description of the design and future directions of this infrastructure. Graphite uses the polyhedral model as the internal representation (GPOLY). The plan is to create a polyhedral compilation package (PCP) that will provide loop optimization and analysis capabilities to GCC. This package will be separated from GIMPLE via an interface language that is restricted to express only what GPOLY can represent. The interface language is a set of data structures that encodes the control flow and memory accesses of a code region. A syntax for the language is also defined to facilitate debugging and testing

Concurrent loss of MLH1, PMS2 and MSH6 immunoexpression in digestive system cancers indicating a widespread dysregulation in DNA repair processes

Immunohistochemical analysis of mismatch repair (MMR) protein expression is widely used to identify tumors with a deficient MMR (dMMR). MMR proteins (MLH1/PMS2 and MSH2/MSH6) work as functional heterodimers, which usually leads to the loss of expression in only one functional MMR heterodimer. Recently, there have been studies showing the simultaneous loss of immunoexpression in proteins of both heterodimers. Yet, this phenomenon has been rarely investigated. In this study, we retrospectively considered cases of different digestive system cancers (gastric cancer, ampullary cancer, small bowel cancer, colorectal cancer), which were immunohistochemically tested for dMMR within a 4-year period at our university hospital (n=352). Of the 103 cases showing dMMR, 5 cases (1.4% of all, 5.1% of dMMR cases) showed a concurrent loss of MLH1, PMS2 and MSH6 immunoexpression, whereas in the other 98 dMMR cases only one MMR heterodimer was affected. MLH1-/PMS2-/MSH6- cancer cases almost arose throughout the entire digestive tract: from the gastric antrum to the left colic flexur. To provide a comprehensive molecular characterization of this MLH1-/PMS2-/MSH6- immunophenotype, tumors were analyzed for microsatellite instability, MLH1 promotor hypermethylation and BRAF exon 15 status. Furthermore, we performed next-generation sequencing focusing on genes related to DNA repair. Here, we could detect pathogenic germline variants as well as multiple sporadic mutations in different genes involved in MMR and homologous recombination repair (HRR) respectively. The affected MMR/HRR-related genes were: ATM, BARD1, BRCA1, CDK12, CHEK1, CHEK2, FANCA, MLH1, MSH6, PALB2, TP53. Considering the biologic function of HRR/MMR proteins as potential drug targets and the low frequency of most of these mutations in digestive system cancers in general, their common occurrence in our MLH1-/PMS2-/MSH6- cases seems to be even more noteworthy, highlighting the need for recognition, awareness and further investigation of this unusual IHC staining pattern

Sömmerung von Karpfenteichen: Erprobung von Möglichkeiten zur Sömmerung von Karpfenteichen unter Berücksichtigung förderrechtlicher und naturschutzfachlicher Aspekte

Im Rahmen des Forschungsprojektes „Sömmerung von Teichen“ wurden Möglichkeiten geprüft zur Wiedereinführung der Sömmerung mit Feldfruchtanbau auf Teilflächen des Teiches unter den heutigen ökonomischen Rahmenbedingungen. Diese Schriftenreihe informiert über die Ergebnisse der Prüfung. Die Sömmerung ist eine Form der Teichbewirtschaftung, mit der auf den sommerlichen Wassermangel reagiert und ein Beitrag zur Fischkrankheitsbekämpfung geleistet werden kann. Auf tragfähigen Böden können Nutzpflanzen angebaut werden. Bei ungünstigen Bodenverhältnissen hat der Anbau von Blühmischungen oder das Zulassen von Brachflächen auf Teilen des Teichbodens günstige Effekte auf die Biodiversität, insbesondere den Insektenschutz und die Entwicklung von seltenen Pionierpflanzengesellschaften. Die Veröffentlichung richtet sich an Teichwirte, Vertreter von Fachbehörden, des Naturschutzes und die breite Öffentlichkeit. Redaktionsschluss: 28.02.202

ALK, NUT, and TRK do not play relevant roles in gastric cancer — results of an immunohistochemical study in a large series

ALK, NUT, and TRK are rare molecular aberrations that are pathognomonic for specific rare tumors. In low frequencies, however, they are found in a wide range of other tumor entities. This study aimed to investigate the frequency, association with clinicopathological characteristics, and prognosis of the immunohistochemical expressions of ALK, NUT, and TRK in 477 adenocarcinomas of the stomach and gastroesophageal junction. Seven cases (1.5%) showed an expression of TRK. In NGS, no NTRK fusion was confirmed. No case with ALK or NUT expression was detected. ALK, NUT, and NTRK expression does not seem to play an important role in gastric carcinomas

Comprehensive immunohistochemical study of the SWI/SNF complex expression status in gastric cancer reveals an adverse prognosis of SWI/SNF deficiency in genomically stable gastric carcinomas

SIMPLE SUMMARY: This study aimed to investigate the clinical relevance of immunohistochemical expression of proteins of the SWI/SNF complex, SMARCA2, SMARCA4 SMARCB1, ARID1A, ARID1B, and PBRM1 in 477 adenocarcinomas of the stomach and gastroesophageal junction. Additionally, the tumors were classified immunohistochemically in analogy to The Cancer Genome Atlas (TCGA) classification. Overall, 32% of cases demonstrated aberrant expression of the SWI/SNF complex. SWI/SNF aberration emerged as an independent negative prognostic factor for overall survival in all patients and in genomically stable patients in analogy to TCGA. In conclusion, determination of SWI/SNF status could be suggested in routine diagnostics in genomically stable tumors to identify patients who might benefit from new therapeutic options. ABSTRACT: The SWI/SNF complex has important functions in the mobilization of nucleosomes and consequently influences gene expression. Numerous studies have demonstrated that mutations or deficiency of one or more subunits can have an oncogenic effect and influence the development, progression, and eventual therapy resistance of tumor diseases. Genes encoding subunits of the SWI/SNF complex are mutated in approximately 20% of all human tumors. This study aimed to investigate the frequency, association with clinicopathological characteristics, and prognosis of immunohistochemical expression of proteins of the SWI/SNF complexes, SMARCA2, SMARCA4 SMARCB1, ARID1A, ARID1B, and PBRM1 in 477 adenocarcinomas of the stomach and gastroesophageal junction. Additionally, the tumors were classified immunohistochemically in analogy to The Cancer Genome Atlas (TCGA) classification. Overall, 32% of cases demonstrated aberrant expression of the SWI/SNF complex. Complete loss of SMARCA4 was detected in three cases (0.6%) and was associated with adverse clinical characteristics. SWI/SNF aberration emerged as an independent negative prognostic factor for overall survival in genomically stable patients in analogy to TCGA. In conclusion, determination of SWI/SNF status could be suggested in routine diagnostics in genomically stable tumors to identify patients who might benefit from new therapeutic options

Sömmerung von Karpfenteichen - Teil 2: Erprobung von Möglichkeiten zur Sömmerung von Karpfenteichen unter Berücksichtigung förderrechtlicher und naturschutzfachlicher Aspekte

Die Sömmerung von Karpfenteichen war lange Zeit fester Bestandteil der Karpfenteichwirtschaft. Im Zusammenhang mit sinkenden Wasserdargeboten rückt die Sömmerung erneut in den Blick. Der Bericht beschreibt die Ergebnisse von Versuchen zur Teichsömmerung. Er richtet sich an alle interessierte Teichwirte. Die Sömmerung kann heute aus hydrologischen, betriebswirtschaftlichen oder seuchenbiologischen Gründen durchaus wieder sinnvoll sein. Durch die Sömmerung von Karpfenteichen entstehen als Nebeneffekt naturschutzfachlich hochwertigste Habitate. Auf Sömmerungsflächen entwickelt sich eine besonders schützenswerter Flora und Fauna. Das Heft ergänzt die Schriftenreihe des LfULG, Heft 8/2021, „Sömmerung von Karpfenteichen“. Redaktionsschluss: 30.11.202